Phone : +91-3222-283756
Location : NB / FF / 6, CRF

Professor-In-Charge
Prof. Amit Kumar Das
Department of Biotechnology
amitk@hijli.iitkgp.ernet.in
+91-3222-283756

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Objectives

  • MALDI ToF/ToF (Matrix Assisted Laser Desorption Ionization Time of Flight/ Time of Flight) Ultraflextreme instrument and it is used for the determination of the molecular mass is an mass spectrometric of different chemical and biomolecules such as protein, peptide, polymers, organic & inorganic molecules etc.
  • This Intrument with special facility to determine denovo protein sequence, peptide mass fingerprint(PMF). This Instrument is coupled with a Nano Lc (Liquid Chromatography) for PMF, denovo protein sequence etc.

 

  • 1. Accurate mass determination.

            2. Peptide mass fingerprint.

3.Identification of microorganism using biotyper.

4.Tissue imaging using image prep.

5. Lipid identification using TLC MALDI.




People


Prof. Amit Kumar Das
Professors-In-Charge
Department of Biotechnology
amitk@hijli.iitkgp.ernet.in
+91-3222-283756
Mr. Bidhan Chandra Sing
Technical Staff
Central Research Facility
abc3@xyz.com
+91-3222-282486

Equipments


MALDI ToF/ToF (Time of Flight/Time of Flight) Utraflextreme

  • Name: MALDI ToF/ToF;  
  • Model: Ultraflextreme  
  • Manufacturer details :  BRUKER DALTONIK GmbH  Life Sciences  Fahrenheitstr. 4, D-28359 Bremen Germany , SALES CONTACT
    phone: +49 (421) 2205-0, SERVICE CONTACT
    phone: +49 (421) 2205 350
  • Year of Instalation: 2014

Utility and Working Principal

 

Sample Details

     Different Type of samples (Protein, peptide,polymer,chemical molecules ) are mixed with appropiate matrix (eg. SA, HCCA,DHB,etc) for crystallization before data acquisition.

Lights
Sample Title

Fig: True spectra of Maldi Tof/Tof  PMF

1.  Prof. Amit Kumar Das                            Biotechnology                         Prof.-In charge

2.  Prof. Arindam Mandal                            Bioscience                               Jt. Prof.-In charge

3.  Prof. Ananta kr. Ghosh                           Biotechnology                         Member                      

4.  Prof. Sudip Kr. Ghosh                            Biotechnology                         Member

5.  Prof. M.K. Maiti                                      Biotechnology                         Member

6.  Prof. S. Dey                                             Biotechnology                         Member

7.  Prof. Amit Basak                                     Chemistry                                Member

8.  Prof. Swagata Dasgupta                          Chemistry                                Member

9.  Prof. Koel Chaudhury                             SMST                                      Member

10.  Prof. M. Mandal                                    SMST                                      Member

11.  Prof. S. Dhara                                        SMST                                      Member

12.  Prof. Nikhil K. Singha                           RTC                                        Member


Publications

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NanoLC MALDI MS/MS based quantitative metabolomics reveals the alteration of membrane biogenesis in oral cancer. RSC Advances, 6(67), pp.62420-62433. ( Journal)

Authors : Bag, S., Dutta, D., Chaudhary, A., Sing, B.C., Banerjee, R., Pal, M., Paul, R.R., Basak, A., Das, A.K., Ray, A.K. and Chatterjee.
Name of the Journal : NanoLC MALDI MS/MS based quantitative metabolomics reveals the alteration of membrane biogenesis in oral cancer. RSC Advances, 6(67), pp.62420-62433. (2016)

Contact Us

Contact

Location: Central Research Facility

        Lab. Phone: 03222 82486

 

Professor-in-charge: Prof. Amit Kumar Das

Email  :  amitk@.iitkgp.ernet.in

Phone (Chmbr.):  03222 83756

 

Lab.Staff: Mr. Bidhan Chandra Sing

Email: bidhanbotany@gmail.com




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