Phone : +91-3222-283760
Location : NB / FF / 10, CRF

Professor-In-Charge
Prof. Satyahari Dey
Department of Biotechnology
sdey@hijli.iitkgp.ernet.in
+91-3222-283760

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Objectives

To determine the accurate mass of several biomolecules and/or synthetic compounds with their diode array detection patern and structural information through fragmentation study. The high sensitive LC-MS/MS are routinely used for mass measurement of flavonoids, alkaloids, phenolics, peptides, drugs, pesticides, terpenoids, and some synthetic compounds with the mass range of 10-2000 Dalton.




People


Prof. Satyahari Dey
Professors-In-Charge
Department of Biotechnology
sdey@hijli.iitkgp.ernet.in
+91-3222-283760
Dr. Samiran Sona Gauri
Technical Staff
Central Research Facility
asmi04@gmail.com
+91-3222-282499

Equipments


LIQUID CHROMATOGRAPHY AND TENDEM MASS SPECTROMETRY

Waters 2695 Separation Module with P.D.A. Detector and Automated Fraction Collector. Quattro microTM API with Mass-Lynx 4.1 upgraded software

Manufacturer details:WATERS, USA

Purchase source (funding):Institute

Year of installation: September, 2008. 

Utility and Working Principal

Liquid chromatography - tandem mass spectrometry (LC-MS/MS, or alternatively HPLC-MS/MS) is a technique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis (parent and daughter ion) capabilities of mass spectrometry. 
This LC-MS/MS is very much useful to determine the accurate mass of several biomolecules and or synthetic compound with their diode array detection pattern. The high sensitive LC-MS/MS are routinely used for accurate mass measurement of flavonoids, alkaloids, phenolics, peptides, drugs, pesticides, terpenoids, and some synthetic compound with the mass range of 10-2000 Dalton. Detailed structural information may be obtained by fragmentation spectra from MS/MS experiments which can be used for identification purpose, metabolic profiling, structure elucidation and peptide sequencing.


Mass spectrometers operate by converting the analyte molecules to a charged (ionised) state, with subsequent analysis of the ions and any fragment ions that are produced during the ionisation process, on the basis of their mass to charge ratio (m/z). Ion source is an important component for any kind of MS analysis, to ionize intact molecules, the ion source could be APCI (Atmospheric Pressure Chemical Ionization), ESI (Electronspray Ionization), etc. The choice of ion source also depends on the chemical nature of the analyte of interest i.e. polar or non-polar. The major advantages of this technology include sensitivity, specificity and precision as analysis is done at molecular level. Also, structural details of the analyte can be deciphered.

The impurities in the sample like any colloidal particle, sugar moiety, salt, buffers, etc. may cause ion suppression as well as damage the detector.

Sample Details

Lights
Sample Title

Publications

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Design and synthesis of enediyne-based peptide with selective peptide-cleaving activity ( Journal)

Authors : Snigdha Roy, and Amit Basak
Name of the Journal : Design and synthesis of enediyne-based peptide with selective peptide-cleaving activity (2010)

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Novel Biotransformation Route for Tannic Acid biotransformation and Its Effect on Biopolymer Synthesis in Azotobacter sp. strain SSB81 ( Journal)

Authors : Samiran S. Gauri, Santi M. Mandal, Sanghamitra Atta, Satyahari Dey, Bikas R. Pati,
Name of the Journal : Novel Biotransformation Route for Tannic Acid biotransformation and Its Effect on Biopolymer Synthesis in Azotobacter sp. strain SSB81 (2013)

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Biotransformation of p?coumaric acid and 2,4?dichlorophenoxy acetic acid by Azotobacter sp. strain SSB81. ( Journal)

Authors : Samiran S. Gauri, Santi M. Mandal, Satyahari Dey & Bikas R. Pati.
Name of the Journal : Biotransformation of p?coumaric acid and 2,4?dichlorophenoxy acetic acid by Azotobacter sp. strain SSB81. (2012)

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Photoremovable protecting groups as controlled release device for sex pheromone. ( Journal)

Authors : Sanghamitra Atta, Mohammed Ikbal, Nishitha Boda, Samiran S. Gauri and N.D. Pradeep Singh.
Name of the Journal : Photoremovable protecting groups as controlled release device for sex pheromone. (2013)

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Exploring the scope of Bergman Cyclization mediated cascade reaction of alkenyl enediynes: synthesis of [5] helicene and amino acid appended helicenes. ( Journal)

Authors : Snigdha Roy and Amit Basak.
Name of the Journal : Exploring the scope of Bergman Cyclization mediated cascade reaction of alkenyl enediynes: synthesis of [5] helicene and amino acid appended helicenes. (2010)

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Biotransformation of betanidin and flavonoids by a betanidin glycosyltransferase ( Journal)

Authors : Amaranthus tricolor L. Shibendu Sekhar Das, Samiran S. Gauri, Biswapriya Biswavas Misra, Mousumi Biswas, Satyahari Dey.
Name of the Journal : Biotransformation of betanidin and flavonoids by a betanidin glycosyltransferase (2007)

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Sweetmeat waste fractions as suitable organic carbon source for biological sulfate reduction. ( Journal)

Authors : Bidus Kanti Das, Samiran S. Gauri, Jayanta Bhattacharya.
Name of the Journal : Sweetmeat waste fractions as suitable organic carbon source for biological sulfate reduction. (2013)

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Identification and Structural Characterization of a Pro-apoptotic Cyclic Octapeptide from Somatic Seedlings of Santalum album ( Journal)

Authors : L. Abheepsa Mishra, Samiran Sona Gauri, Sourav Kanti Mukhopadhyay, Soumya Chatterjee, Shibendu Sekhar Das, , Moumita Patra, Satyahari Dey.
Name of the Journal : Identification and Structural Characterization of a Pro-apoptotic Cyclic Octapeptide from Somatic Seedlings of Santalum album (2014)

Contact Us

Contact Details:             Prof. Satyahari Dey (PIC),

                      dept. of Biotechnology, sdey@hijli.iitkgp.ernet.in

                                     Office Tel No-283760 (03222)

                                  Dr. Samiran Sona Gauri (Technical Staff).

                                     Lab Tel No-282499 (03222)




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