Pulsed Electron Spin Paramagnetic Resonance Laboratory

Phone : +91-3222-283804

Location : OB / GF / *, CRF

Facilitator : Dr. Debamalya Banerjee, Physics
Email: debamalya@phy.iitkgp.ernet.in, Contact:+91-3222-283804


Detection of unpaired electron spins and their interaction with neighbouring  lattice. This instrument is capable of detecting electron spin concentration as low as 1017/cc.


Dr. Debamalya Banerjee
Mr. Arijit Maity
Technical Staff
Central Research Facility

Equipment Details

Pulsed Electron paramagnetic resonance (EPR) Spectrometer (X-band)

Manufacturer: Bruker.

Model No.: ELEXSYS 580

Year of installation: 2016


 Microwave frequency range  X-band (8-12 GHz)
 Magnetic field scan range  -1 to 1 Tesla
 Temperature range  4.2 to 160 K and room temperature
 Maximum microwave power  150 mW
  Modulation field amplitude  10 G max
 Modulation field frequency  10-100KHz
 Quadrature detection  Real and imaginary
 Sample state  See details afterwards


Utility and Working Principal

Any unpaired spin has a net magnetic moment. EPR spectroscopy exploits the net magnetic moments of systems having unpaired electron spins. Spin hamiltonian of an unpaired electron in an external magnetic can be written as:

H = ge ????B B0 .?

= ge ????B B0 Sz (B0 ? ? direction)

= ge ????B B0 Ms ­(MS=±1/2)……………………………..(1)

Here ge (=2.20023) is g-factor of free electron, ????B (=9.27?10-24 J/T) is Bohr’s magneton,

Ms is spin projection quantum number. If the electron is bound e.g. an electron captured in

a dangling bond state, then ge would be replaced by g.

The energy difference between two Zeeman energy levels is, ????E= g ????B B0. Now, if a microwave of frequency ???? is applied, then a resonant absorption of microwave will occur if energy of microwave photon becomes equal to energy difference between the two Zeeman levels i.e.,

h????= g ????B B0 ­; with selection rule ???? MS=±1 for ESR transition.

At thermal equilibrium the fractional population of upper and lower spin level can calculated by Boltzmann’s distribution law as,


Where N is total number of spins in the system, Nlow and Nup are spin population in upper and lower spin energy level, K is Boltzmann’s constant, and T is temperature.

Now microwave absorption intensity (IESR) is proportional to the population difference between two energy levels [5] i.e.

IESRΔN=Nlow-Nup≈N.gμBB02KT, whenΔEKT?1……………(3)

Since IESRis proportional to resonant magnetic field B0 (and hence resonant frequency) and inversely proportional to temperature, the signal to noise ratio can be improved by measuring ESR at low temperature and at high microwave frequency. Equations (2) and (3) is only valid for isolated spins such as dangling bond states which are not coupled.

Sample Details

A. For Solid Sample:

i) Sample has to be filled in glass capillary tube with both side sealed.


ii) Sample has to be filled in standard quartz (NMR/EPR) tube (250 mm

long and diameter < 4 mm).

B. For Liquid Sample:

i) Non-aqueous (Non H-bonded): Sample has to be filled in standard EPR

quartz tube / glass capillary with both side sealed (if both side sealing is not

possible, then other side has to be sealed well with paraffin wax).

ii) Aqueous/H-bonded: Sample has to be filled in thin glass capillary with

both side sealed (if both side sealing is not possible, then other side has to

be properly sealed with paraffin wax). Total sample volume must be less than

10 microlitter.

Reason: Aqueous/H-bonded samples absorb lot of microwaves. We cannot

tune the microwave bridge with excess sample volume due to this absorption.

****** If above guideline is not followed, then we might not be able to

accept samples.

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