Real Time PCR Laboratory

Phone : +91-3222-283762

Location : NB / FF / 4, CRF

Facilitator : Dr. Ritobrata Goswami, School of Bio Science
Email: ritobrata.goswami@iitkgp.ac.in, Contact:+91-3222-214570



Objectives

Quantification of RNA transcript level at a certain time/ condition in a cell. Primers based specific pathogens at very low threshold limit. Evaluation of the primers for specificity to the target organism, A conventional diagnostic protocol.

People

Dr. Ritobrata Goswami
Facilitator
School of Bio Science
ritobrata.goswami@iitkgp.ac.in
+91-3222-214570

Equipment Details

ABI PRISM 7000 Sequence Detection Systems

ABI PRISM 7000 Sequence Detection Systems, Applied Biosystem, USA.

Utility and Working Principal

Real-time PCR is a thermal cycler with the capacity to fluorescence each sample with a beam of light of at least one specified wavelength and detect the fluorescence emitted by the excited fluorophore.


Sample Details

  • Samples will not be analyzed unless complete information is provided.

  • Must bring your DNA samples, primers, buffer, enzymes and PCR water to set up the reaction.

  • The user must bring samples to CRF on the date and time of analysis.

  • Sequencing data will be provided in CD or as e-mail attachment.



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